RELATIONSHIP BETWEEN IN VITRO AND SEEDLING SELECTION FOR RESISTANCE TO SPOT BLOTCH (Bipolaris sorokiniana) IN WHEAT
Keywords:
Bipolaris sorokiniana, Triticum aestivum, toxic filtrates, tissue culture.Abstract
Twelve wheat (Triticum aestivum L.) genotypes were assessed, in vitro and in vivo, for resistance to spot
blotch induced by Bipolaris sorokiniana, with the objective of verify correlation between calli and seedling responses. Toxic
filtrates were obtained from two fungai isolates and added to the culture mediam where the wheat callus developed; spore
suspensions were also produced and applied to wheat seedlings. The callus reaction was quantified by the difference between
growth of those exposed to the filtrates and the untreated cheks. The response of the seedlings was measured by the percentage
of necrotic lesions present on the leaves. A smaller callus growth corresponded to a higher percentage of lesions on the
seedling leaves.
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