Eucalyptus staigeriana essential oil can control downy mildew in grapevine
Resumen
Downy mildew (Plasmopara viticola) is the primary disease in viticulture worldwide, and your control requires synthetic fungicides applications to avoid quality and yield loss in the grapevines. However, alternatives to reduce synthetic fungicides are needed to ensure the consumer’s health and the environment. Essential oils (EOs) are amongst the most promising natural plant protection alternatives because of their antifungal properties on several crop diseases. The present study objective was to determine the effect of Eucalyptus staigeriana EO in vitro and in vivo against P. viticola. The EO exhibited the highest activity in vitro, inhibiting 90% of the incidence and severity of disease caused by P. viticola in leaves of grapevines in the greenhouse. In the field (in vivo), treatment with EO could not control the disease; however, treatment with EO in consortium with conventional treatment reduced approximately 50% of the incidence and more than 90% of the severity of downy mildew disease in leaves, decreasing the application of synthetic fungicides by 50%.
Descargas
Citas
BROOKER, M. I. H.; KEING, D. A. Field guide to Eucalyptus. Third edition. Melbourne: Bloomings Book, 2006.
BUFFARA, C. R. S. et al. Elaboration and validation of a diagrammatic scale to assess downy mildew severity in grapevine. Ciência Rural, v. 44, n.8, 2014. DOI: https://doi.org/10.1590/0103-8478cr20131548. DOI: https://doi.org/10.1590/0103-8478cr20131548
CHEN, M. et al. Forecasting severe grape downy mildew attacks using machine learning. Plos one, v. 15, n. 3, 2020. DOI: https://doi.org/10.1371/journal.pone.0230254. DOI: https://doi.org/10.1371/journal.pone.0230254
CHEN, W. J. et al. At least two origins of fungicide resistance in grapevine downy mildew populations. Applied and environmental microbiology, v.73, p. 5162–5172, 2007. DOI: https://doi.org/10.1128/AEM.00507-07. DOI: https://doi.org/10.1128/AEM.00507-07
DAGOSTIN, S. et al. Are there alternatives to copper for controlling grapevine downy mildew in organic viticulture? Crop protection, v. 30, n. 7, p. 776–788, 2011. DOI: https://doi.org/10.1016/j.cropro.2011.02.031. DOI: https://doi.org/10.1016/j.cropro.2011.02.031
DHAKAD, A. K. et al. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review. Journal of the Science of Food and Agriculture, v. 98, p. 833-848, 2018. DOI: https://doi.org/10.1002/jsfa.8600. DOI: https://doi.org/10.1002/jsfa.8600
FALASCA, A. et al. GC-MS analysis of the essential oils of Juniperus communis L. berries growing wild in the Molise region: Seasonal variability and in vitro antifungal activity. Biochemical Systematics and Ecology, v. 69, p. 166-175, 2016. DOI: https://doi.org/10.1016/j.bse.2016.07.026. DOI: https://doi.org/10.1016/j.bse.2016.07.026
FAWKE, S.; DOUMANE, M.; SCHORNACK, S. Oomycete interactions with plants: infection strategies and resistance principles. Microbiology and Molecular Biology Reviews, v. 79, p. 263-280, 2015. DOI: https://doi.org/10.1128/MMBR.00010-15. DOI: https://doi.org/10.1128/MMBR.00010-15
FRÖBEL, S.; ZYPRIAN, E. Colonization of different grapevine tissues by Plasmopara viticola - A histological study. Frontiers in Plant Science, v. 10, p. 951, 2019. DOI: https://doi.org/10.3389/fpls.2019.00951. DOI: https://doi.org/10.3389/fpls.2019.00951
GESSLER, C.; PERTOT, I.; PERAZZOLLI, M. Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathologia Mediterranea, v. 50, n. 1, 2011.
GISI, U.; SIEROTZKI, H. Fungicide modes of action and resistance in downy mildews. European Journal of Plant Pathology, v. 122, n. 1, p.157–67, 2008. DOI: https://doi.org/10.1007/s10658-008-9290-5. DOI: https://doi.org/10.1007/s10658-008-9290-5
KASSEMEYER, H-H.; GADOURYM, D. M.; HILL, G. Downy Mildew. In Compendium of Grape Diseases, Disorders, and Pests. 2nd Edn., eds W. Wilcox, W. D. Gubler, and K. J. Uyemoto (St. Paul: APS Press), p. 46–52, 2015.
KIM, K-H.; KABIR, E.; JAHAN, S. A. Exposure to pesticides and the associated human health effects. Science of the total Environment, v. 575, p. 525–535, 2017. DOI: https://doi.org/10.1016/j.scitotenv.2016.09.009. DOI: https://doi.org/10.1016/j.scitotenv.2016.09.009
LA TORRE, A. et al. Evaluation of essential plant oils for the control of Plasmopara viticola. Journal of essential oil research, v. 26, n. 4, p. 282–91, 2014. DOI: https://doi.org/10.1080/10412905.2014.889049. DOI: https://doi.org/10.1080/10412905.2014.889049
MACEDO, I. T. F. et al. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Veterinary Parasitology, v. 173, p. 93-98, 2010. DOI: https://doi.org/10.1016/j.vetpar.2010.06.004. DOI: https://doi.org/10.1016/j.vetpar.2010.06.004
MASSI, F. et al. Fungicide resistance evolution and detection in plant pathogens: Plasmopara viticola as a case study. Microorganisms, v. 9, n. 1, p. 119, 2021. DOI: https://doi.org/10.3390/microorganisms9010119. DOI: https://doi.org/10.3390/microorganisms9010119
NAZZARO, F. et al. Essential oils and antifungal activity. Pharmaceuticals, v. 10, n. 4, p. 86, 2017. DOI: https://doi.org/10.3390/ph10040086. DOI: https://doi.org/10.3390/ph10040086
PANDEY, A. K. et al. Essential oils: sources of antimicrobials and food preservatives. Frontiers in Microbiology, v. 7, n. 2161, 2017. DOI: https://doi.org/10.3389/fmicb.2016.02161. DOI: https://doi.org/10.3389/fmicb.2016.02161
PEDROTTI, C. et al. Alternative control of grape rots by essential oils of two Eucalyptus species. Journal of Science of Food and Agriculture, v. 99, n. 14, p. 6552-6561, 2019. DOI: https://doi.org/10.1002/jsfa.9936. DOI: https://doi.org/10.1002/jsfa.9936
PEDROTTI, C. et al. Essential oil as sustainable alternative for diseases management of grapes in postharvest and in vineyard and its influence on wine. Journal of Environmental Science and Health, Part B, v. 56, n. 1, p. 73-81, 2020. DOI: https://doi.org/10.1080/03601234.2020.1838827. DOI: https://doi.org/10.1080/03601234.2020.1838827
PERTOT, I. et al. A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture. Crop Protection, v. 97, p. 70–84, 2017. DOI: https://doi.org/10.1016/j.cropro.2016.11.025. DOI: https://doi.org/10.1016/j.cropro.2016.11.025
RIENTH, M. et al. Oregano essential oil vapour prevents Plasmopara viticola infection in grapevine (Vitis vinifera) and primes plant immunity mechanisms. Plos one, v. 14, n. 9, 2019. DOI: https://doi.org/10.1371/journal.pone.0222854. DOI: https://doi.org/10.1371/journal.pone.0222854
ROMANAZZI, G. et al. Recent advances on the use of natural and safe alternatives to conventional methods to control postharvest gray mold of table grapes. Postharvest Biology and Technology, v. 63, p. 141–147, 2012. DOI: https://doi.org/10.1016/j.postharvbio.2011.06.013. DOI: https://doi.org/10.1016/j.postharvbio.2011.06.013
TANG, X. et al. Antifungal activity of essential oil compounds (geraniol and citral) and inhibitory mechanisms on grain pathogens (Aspergillus flavus and Aspergillus ochraceus). Molecules, v. 23, p. 2108, 2018. DOI: https://doi.org/10.3390/molecules23092108. DOI: https://doi.org/10.3390/molecules23092108
TOMAZONI, E. Z. et al. Screening for inhibitory activity of essential oils on fungal tomato pathogen Stemphylium solani Weber. Biocatalysis and Agricultural Biotechnology, v. 16, p. 364-372, 2018. DOI: https://doi.org/10.1016/j.bcab.2018.08.012. DOI: https://doi.org/10.1016/j.bcab.2018.08.012
TOMAZONI, E. Z. et al. In vitro and in vivo activity of essential oils extracted from Eucalyptus staigeriana, Eucalyptus globulus and Cinnamomum camphora against Alternaria solani Sorauer causing early blight in tomato. Scientia Horticulturae, v. 223, p. 72–77, 2017. DOI: https://doi.org/10.1016/j.scienta.2017.04.033. DOI: https://doi.org/10.1016/j.scienta.2017.04.033
TUREK, C.; STINTZING, F.C. Stability of essential oils: a review. Comprehensive Reviews in Food Science and Food Safety, v. 12, n. 1, p. 40–53, 2013. DOI: https://doi.org/10.1111/1541-4337.12006. DOI: https://doi.org/10.1111/1541-4337.12006
ZAKER, M. Natural plant products as eco-friendly fungicides for plant diseases control- a review. The Agriculturists, v. 14, n. 1, p. 134-141, 2016. DOI: https://doi.org/10.3329/agric.v14i1.29111. DOI: https://doi.org/10.3329/agric.v14i1.29111
ZHENG, S. et al. Citral exerts its antifungal activity against Penicillium digitatum by affecting the mitochondrial morphology and function. Food Chemistry, v. 178, p. 76–81, 2015. DOI: https://doi.org/10.1016/j.foodchem.2015.01.077. DOI: https://doi.org/10.1016/j.foodchem.2015.01.077
Derechos de autor 2021 Pesquisa Agropecuária Gaúcha
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
Los autores declaran que el trabajo no ha sido publicado previamente, ni enviado simultáneamente para su publicación en otra revista y que están de acuerdo con la presentación, el contenido y la transferencia de los derechos de publicación del artículo en cuestión a la revista científica Pesquisa Agropecuária Ga´´ucha - PAG. Los autores asumen toda la responsabilidad por la originalidad del artículo y pueden incurrir en ellos por cualquier cargo que surja de reclamos de terceros en relación con la autoría del artículo. La reproducción completa de los artículos de la revista en otros medios electrónicos de uso gratuito está permitida bajo la licencia Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional.
