Eucalyptus staigeriana essential oil can control downy mildew in grapevine

Keywords: Alternative control, Plasmopara viticola, Eucalypt, Secondary metabolites, Vitis

Abstract

Downy mildew (Plasmopara viticola) is the primary disease in viticulture worldwide, and your control requires synthetic fungicides applications to avoid quality and yield loss in the grapevines. However, alternatives to reduce synthetic fungicides are needed to ensure the consumer’s health and the environment. Essential oils (EOs) are amongst the most promising natural plant protection alternatives because of their antifungal properties on several crop diseases. The present study objective was to determine the effect of Eucalyptus staigeriana EO in vitro and in vivo against P. viticola. The EO exhibited the highest activity in vitro, inhibiting 90% of the incidence and severity of disease caused by P. viticola in leaves of grapevines in the greenhouse. In the field (in vivo), treatment with EO could not control the disease; however, treatment with EO in consortium with conventional treatment reduced approximately 50% of the incidence and more than 90% of the severity of downy mildew disease in leaves, decreasing the application of synthetic fungicides by 50%.

Downloads

Download data is not yet available.

References

BROOKER, M. I. H.; KEING, D. A. Field guide to Eucalyptus. Third edition. Melbourne: Bloomings Book, 2006.

BUFFARA, C. R. S. et al. Elaboration and validation of a diagrammatic scale to assess downy mildew severity in grapevine. Ciência Rural, v. 44, n.8, 2014. DOI: https://doi.org/10.1590/0103-8478cr20131548. DOI: https://doi.org/10.1590/0103-8478cr20131548

CHEN, M. et al. Forecasting severe grape downy mildew attacks using machine learning. Plos one, v. 15, n. 3, 2020. DOI: https://doi.org/10.1371/journal.pone.0230254. DOI: https://doi.org/10.1371/journal.pone.0230254

CHEN, W. J. et al. At least two origins of fungicide resistance in grapevine downy mildew populations. Applied and environmental microbiology, v.73, p. 5162–5172, 2007. DOI: https://doi.org/10.1128/AEM.00507-07. DOI: https://doi.org/10.1128/AEM.00507-07

DAGOSTIN, S. et al. Are there alternatives to copper for controlling grapevine downy mildew in organic viticulture? Crop protection, v. 30, n. 7, p. 776–788, 2011. DOI: https://doi.org/10.1016/j.cropro.2011.02.031. DOI: https://doi.org/10.1016/j.cropro.2011.02.031

DHAKAD, A. K. et al. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review. Journal of the Science of Food and Agriculture, v. 98, p. 833-848, 2018. DOI: https://doi.org/10.1002/jsfa.8600. DOI: https://doi.org/10.1002/jsfa.8600

FALASCA, A. et al. GC-MS analysis of the essential oils of Juniperus communis L. berries growing wild in the Molise region: Seasonal variability and in vitro antifungal activity. Biochemical Systematics and Ecology, v. 69, p. 166-175, 2016. DOI: https://doi.org/10.1016/j.bse.2016.07.026. DOI: https://doi.org/10.1016/j.bse.2016.07.026

FAWKE, S.; DOUMANE, M.; SCHORNACK, S. Oomycete interactions with plants: infection strategies and resistance principles. Microbiology and Molecular Biology Reviews, v. 79, p. 263-280, 2015. DOI: https://doi.org/10.1128/MMBR.00010-15. DOI: https://doi.org/10.1128/MMBR.00010-15

FRÖBEL, S.; ZYPRIAN, E. Colonization of different grapevine tissues by Plasmopara viticola - A histological study. Frontiers in Plant Science, v. 10, p. 951, 2019. DOI: https://doi.org/10.3389/fpls.2019.00951. DOI: https://doi.org/10.3389/fpls.2019.00951

GESSLER, C.; PERTOT, I.; PERAZZOLLI, M. Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathologia Mediterranea, v. 50, n. 1, 2011.

GISI, U.; SIEROTZKI, H. Fungicide modes of action and resistance in downy mildews. European Journal of Plant Pathology, v. 122, n. 1, p.157–67, 2008. DOI: https://doi.org/10.1007/s10658-008-9290-5. DOI: https://doi.org/10.1007/s10658-008-9290-5

KASSEMEYER, H-H.; GADOURYM, D. M.; HILL, G. Downy Mildew. In Compendium of Grape Diseases, Disorders, and Pests. 2nd Edn., eds W. Wilcox, W. D. Gubler, and K. J. Uyemoto (St. Paul: APS Press), p. 46–52, 2015.

KIM, K-H.; KABIR, E.; JAHAN, S. A. Exposure to pesticides and the associated human health effects. Science of the total Environment, v. 575, p. 525–535, 2017. DOI: https://doi.org/10.1016/j.scitotenv.2016.09.009. DOI: https://doi.org/10.1016/j.scitotenv.2016.09.009

LA TORRE, A. et al. Evaluation of essential plant oils for the control of Plasmopara viticola. Journal of essential oil research, v. 26, n. 4, p. 282–91, 2014. DOI: https://doi.org/10.1080/10412905.2014.889049. DOI: https://doi.org/10.1080/10412905.2014.889049

MACEDO, I. T. F. et al. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Veterinary Parasitology, v. 173, p. 93-98, 2010. DOI: https://doi.org/10.1016/j.vetpar.2010.06.004. DOI: https://doi.org/10.1016/j.vetpar.2010.06.004

MASSI, F. et al. Fungicide resistance evolution and detection in plant pathogens: Plasmopara viticola as a case study. Microorganisms, v. 9, n. 1, p. 119, 2021. DOI: https://doi.org/10.3390/microorganisms9010119. DOI: https://doi.org/10.3390/microorganisms9010119

NAZZARO, F. et al. Essential oils and antifungal activity. Pharmaceuticals, v. 10, n. 4, p. 86, 2017. DOI: https://doi.org/10.3390/ph10040086. DOI: https://doi.org/10.3390/ph10040086

PANDEY, A. K. et al. Essential oils: sources of antimicrobials and food preservatives. Frontiers in Microbiology, v. 7, n. 2161, 2017. DOI: https://doi.org/10.3389/fmicb.2016.02161. DOI: https://doi.org/10.3389/fmicb.2016.02161

PEDROTTI, C. et al. Alternative control of grape rots by essential oils of two Eucalyptus species. Journal of Science of Food and Agriculture, v. 99, n. 14, p. 6552-6561, 2019. DOI: https://doi.org/10.1002/jsfa.9936. DOI: https://doi.org/10.1002/jsfa.9936

PEDROTTI, C. et al. Essential oil as sustainable alternative for diseases management of grapes in postharvest and in vineyard and its influence on wine. Journal of Environmental Science and Health, Part B, v. 56, n. 1, p. 73-81, 2020. DOI: https://doi.org/10.1080/03601234.2020.1838827. DOI: https://doi.org/10.1080/03601234.2020.1838827

PERTOT, I. et al. A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture. Crop Protection, v. 97, p. 70–84, 2017. DOI: https://doi.org/10.1016/j.cropro.2016.11.025. DOI: https://doi.org/10.1016/j.cropro.2016.11.025

RIENTH, M. et al. Oregano essential oil vapour prevents Plasmopara viticola infection in grapevine (Vitis vinifera) and primes plant immunity mechanisms. Plos one, v. 14, n. 9, 2019. DOI: https://doi.org/10.1371/journal.pone.0222854. DOI: https://doi.org/10.1371/journal.pone.0222854

ROMANAZZI, G. et al. Recent advances on the use of natural and safe alternatives to conventional methods to control postharvest gray mold of table grapes. Postharvest Biology and Technology, v. 63, p. 141–147, 2012. DOI: https://doi.org/10.1016/j.postharvbio.2011.06.013. DOI: https://doi.org/10.1016/j.postharvbio.2011.06.013

TANG, X. et al. Antifungal activity of essential oil compounds (geraniol and citral) and inhibitory mechanisms on grain pathogens (Aspergillus flavus and Aspergillus ochraceus). Molecules, v. 23, p. 2108, 2018. DOI: https://doi.org/10.3390/molecules23092108. DOI: https://doi.org/10.3390/molecules23092108

TOMAZONI, E. Z. et al. Screening for inhibitory activity of essential oils on fungal tomato pathogen Stemphylium solani Weber. Biocatalysis and Agricultural Biotechnology, v. 16, p. 364-372, 2018. DOI: https://doi.org/10.1016/j.bcab.2018.08.012. DOI: https://doi.org/10.1016/j.bcab.2018.08.012

TOMAZONI, E. Z. et al. In vitro and in vivo activity of essential oils extracted from Eucalyptus staigeriana, Eucalyptus globulus and Cinnamomum camphora against Alternaria solani Sorauer causing early blight in tomato. Scientia Horticulturae, v. 223, p. 72–77, 2017. DOI: https://doi.org/10.1016/j.scienta.2017.04.033. DOI: https://doi.org/10.1016/j.scienta.2017.04.033

TUREK, C.; STINTZING, F.C. Stability of essential oils: a review. Comprehensive Reviews in Food Science and Food Safety, v. 12, n. 1, p. 40–53, 2013. DOI: https://doi.org/10.1111/1541-4337.12006. DOI: https://doi.org/10.1111/1541-4337.12006

ZAKER, M. Natural plant products as eco-friendly fungicides for plant diseases control- a review. The Agriculturists, v. 14, n. 1, p. 134-141, 2016. DOI: https://doi.org/10.3329/agric.v14i1.29111. DOI: https://doi.org/10.3329/agric.v14i1.29111

ZHENG, S. et al. Citral exerts its antifungal activity against Penicillium digitatum by affecting the mitochondrial morphology and function. Food Chemistry, v. 178, p. 76–81, 2015. DOI: https://doi.org/10.1016/j.foodchem.2015.01.077. DOI: https://doi.org/10.1016/j.foodchem.2015.01.077

Published
2021-12-27
How to Cite
PEDROTTI, C.; FRANZOI, C.; SANDI, R. N.; SANTOS GROHS, D.; SCHWAMBACH, J. Eucalyptus staigeriana essential oil can control downy mildew in grapevine. Pesquisa Agropecuária Gaúcha, v. 28, n. 1, p. 1-14, 27 Dec. 2021.

Most read articles by the same author(s)