EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
Abstract
A Indirect enzyme-linked immunosorbent assay (ELISA) kit was used for diagnosis of foot and mouth disease virus (FMDV) typeS 01, A24, C3 which occured in Rio Grande do Sul State, Southern Brazil during 1984-1993. The samples were randomly selected and tested by. Elisa, Complement Fixation Test (CFT) and in tissue ctilture. Out of 106 samples 78 (73:5%) were positive by Elisa and 39(36.8 %) were found positive in CFT, when original suspensions were used. Once_those samples were inoculated onto tissue culture both tests gave similar results, although Elisa picked tip more positive samples during the lst passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The Elisa was more sensitive than and as speci fie as CFT. Elisa and tissue culture together were shown to be a better system for detection of Foot and Mouth Disease (FMD) antigen than CFT.
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