EFFICIENCY OF ANTISERA PRODUCED TO SOFT-ROTTING PECTOBACTERIA IN POTATO
Abstract
Antisera produced against intact and untreated cells of Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) and P chrysanthemi (Pch) were used to index several plants of potatoe that exhibited syntoms of blackleg disease. The stalk samples were better than the root and leave samples for the serological evaluation of Pectobacterium spp. Elowever, only several samples with blacleg syntoms had enough antigens for the visualization in the latex agglutination test. Of 66 plants with blackleg syntoms, collected in the field and indexed by serological methods, only 23 were considered as not typical for Pectobacterium by biochemical tests and did not react positively in the serological tests. Twenty six out of 43 isolates of Pectobacterium spp. were considered as Pca and 17 as Pcc by the biochemical and physiological tests. Flowever, only 17 isolates reacted with the antisera for ? carotovorum (Pca and Pcc) and, not all of them correspond to the previous biochemical characterization. The low number of positive serological reactions with the bacterial isolates indicate that has occurred an involuntary selection of strains for different serotypes. No one of the isolates react with the antisera against Pch. The results allow to conclude that the latex agglutination test can be used for the preliminary identification of bacteria of the genus Pectobacterium, specially when associated with biochemical and physiological tests.
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