ENZYMATIC ACTIVITY OF FLOODED RICE GENOTYPES UNDER DIFFERENT ENVIRONMENTAL CONDITIONS
Abstract
The pre-germinated rice growing system demands genotypes with a higher capacity of initial development in an anaerobic environment. Having in mind the need for better knowing the characteristics of the cultivars El Paso 144, BR IRGA 409, BR IRGA 410, IRGA 416, IRGA 417 and Bluebelle, this paper reports a comparative study of the enzymatic activity of glutamate oxaloacetate transaminase (GOT), esterase (EST), and malate dehydrogenase (MDH), in the rice seedling shoots through electrophoresis. The treatments consisted in germination and development of the seedlings during 14 days at the temperatures of 20, 25 and 30oC under both aerobic and anaerobic conditions. The analysis of the electrophoresis gels leads to conclude that: a) The IRGA 417 seedlings stands out against the other cultivars because of its higher level of activity of the enzymes studied under the anaerobic treatment at the temperature of 20o C; and b) The great variability found in the enzymatic systems studied for the different genotypes and treatments used allow to recommend that studies of genotype x environment interaction use biochemical systems, in order to assist in the search for genotypes that are able to overcome environmental stress.
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