PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
Abstract
Polyclonal antisera against Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) and P chrysanthemi (Pch) were produced from intact, untreated cel Is. Bacterial suspensions with 6 to 7 x CFU/ml (2 ml), emulsified with an equal volume of complete Freund's adjuvant were used as antigens to immunize rabbits. The antigens were injected four times at weekly intervala. The antisera obtained (1:640 titer) were specific against the antigens used and did not exhibit any reaction toward other phytopathogenic bacteria, vírus and fungi, as well as potato, tomato, and tobacco plant extracts. Only antigens presents in the cellular structures of Pca, Pcc and Pch are identified by the antibodies and that the latex agglutination test can be used with di I utions of the antigens in order of 1:1 and 1:10 of the initial concentration (50% T, 550 nm).
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