Genetic characterization of Rhizobia isolated from Lotus corniculatus L.
Abstract
Thirty three Rhizobium sp isolates obtained from birdsfoot trefoil (Lotus corniculatus L.) collected on different regions of Rio Grande do Sul were characterized using Box-PCR. The isolates presented distinct amplification profiles among then, as well as from the isolates recommended nowadays for the production of birdsfoot trefoil inoculants, SEMIA 806 and SEMIA 816. The genetic similarity index, determined by the Jaccard coefficient, ranged from 0.5 to 0.9%, pointing out to a large genetic variability among the isolates. The cluster analysis isolates showed a formation of tree large groups and was not possible to establish a relationship among the amplification profiles, groups formed and geographic origin of the isolates.
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