PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)

Abstract

The effect of Nicotiana glauca and Chrysanthemum vulgaris leave extracts on the humoral response in cattle vaccinated with an inactivated vaccine to bovine Herpesvirus type 5 (BHV-5) were examined. Two inactivated oil-adjuvanted BHV-5 vaccines were prepared with an infectious titre (pre-inactivation) of 10 84DICC/ ml, inactivated with binary ethylenimine (BEI) and emulsified in oil adjuvant. Nicotiana glauca and Chrysanthemum vulgaris extracts were added . (5mg of each extract per dose) as a coadjuvant to one of the vaccines (vaccine B). Two groups of nineteen calves were vaccinated with 3 doses (3 ml) at four week intervals between doses. Neutralizing antibody leveis in sera were determined at different time intervals after vaccination. Thirty days after the administration of the third dose the mean neutralizing antibody titre was 96 for vaccine A and 80 for vaccine B. There was no significant difference between the antibody titres induced by the two vaccines (p>0.05). These results indicate that extracts of these plants had no adjuvant effect on the inactivated BHV-5 vaccine .