MICE VIRULENCE ASSAY OF Pasteurella muttocida STRAINS ISOLATED FROM PIGS

  • SANDRA M. BOROWSKI
  • DAVID E.S.N. BARCELLOS
  • MARISA CARDOSO
Keywords: swine pathology, pneumonia, pleuritis.

Abstract

Virulence of 24 strains of Pasteurella multocida, 13 isolated from pleuritic and pneumonic pig lungs, and 11 from pig lungs obtained from abattoirs was studied. P multocida was cultured in BHI medium incubated at 37° C for 18 h and the number of colony forming units (C FU/ml) determined by inoculation of decimal dilutions on blood agar plates. Dilutions from 10 4 to 10.13 (8,3 x 10 15 to 1 CFU) were inoculated intraperitoneally in mice and the number of deaths for each dilution determined over a 7-day period. Each inoculated strain was recovered from at least two mice. LD50 were determined by the Spearman- Kãrber method. It was observed a wide variation in LD50, ranging from <1 to 10 10.2 CFU. There was no difference in virulence among strains from clinical cases or from abattoir lungs.

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Published
1999-12-30
How to Cite
BOROWSKI, S. M.; BARCELLOS, D. E.; CARDOSO, M. MICE VIRULENCE ASSAY OF Pasteurella muttocida STRAINS ISOLATED FROM PIGS. Pesquisa Agropecuária Gaúcha, v. 5, n. 2, p. 375-379, 30 Dec. 1999.