Sediment and water can act as reservoirs for White spot syndrome virus in shrimp farming

  • Lissandra Souto Cavalli Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do RS https://orcid.org/0000-0001-8531-7362
  • Carolina Reyes Batista Universidade Federal do Rio Grande (FURG)
  • Bruna F.S. Nornberg Universidade Federal do Rio Grande (FURG) https://orcid.org/0000-0002-2383-5137
  • Luis Fernando Marins Universidade Federal do Rio Grande (FURG)
  • Paulo César Abreu Universidade Federal do Rio Grande (FURG)
Keywords: WSSV, Aquaculture, Virus infection

Abstract

Natural viral reservoirs such as water and sediment, in addition to other aquatic organisms may represent an extra route of transmission of the white spot syndrome virus (WSSV). The aim of the present work was to verify the presence of WSSV DNA in the sediment and water in ponds of the white shrimp Litopenaeus vannamei. DNA from sediment and water samples were obtained from four ponds in the same shrimp farm. The presence of viral DNA was tested by nested PCR with WSSV-specific primers. The data revealed the presence of WSSV DNA in the sediment and water samples from two different ponds. The results demonstrate that WSSV can be present in the sediment and water of shrimp farms, acting as important reservoirs for the dissemination of this pathogen. Sanitary measures and prophylaxis should be efficiently employed to avoid future cases of the disease. Monitoring the presence of WSSV in the water and soil of breeding sites can represent an important tool for assessing the potential of viral infection.

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References

ALAVANDI, A.V. et al. Tangential flow ultrafiltration for detection of white spot syndromevirus (WSSV) in shrimp pond water. Journal of Virological Methods v. 218, p. 7–13, 2015. DOI: https://doi.org/10.1016/j.jviromet.2015.03.001

CAVALLI, L.S. et al. Natural occurrence of White spot syndrome virus and Infectious hypodermal and hematopoietic necrosis virus in Neohelice granulata crab. Journal of Invertebrate Pathology v.114, p. 86-88, 2013. DOI: https://doi.org/10.1016/j.jip.2013.06.002

CHANG, P.S.; CHEN, L.J.; WANG, Y.C. The effect of ultraviolet irradiation, heat, pH, ozone, salinity and chemical disinfectants on the infectivity of White spot syndrome baculovirus. Aquaculture v. 166, p. 1-17, 1998. DOI: https://doi.org/10.1016/S0044-8486(97)00238-X

CHANRATCHAKOOL, P.; LIMSUWAN, C. Appllication of PCR and formalin treatment to prevent white spot disease in shrimp. In: FLEGEL, T.W. (Ed). Advances in shrimp Biotechnology. BIOTEC, Bangkok, Thailand, 1998, p.287-289.

CROCKFORD, M. White spot Disease. Australia and New Zeland Standard Diagnostic Procedures, 1-16, 2001.

ENGLAND, L.S.; HOLMES, S.B.; TREVORS, J.T. Review: Persistence of viruses and DNA in soil. World Journal of Microbiology Biotechnology v. 14, p. 163-169, 1998. DOI: https://doi.org/10.1023/A:1008865609698

FEGAN, D.F.; CLIFFORD, H.C. Health management for viral diseases in shrimp farms. In: BROWDY, C.L.; JORY, D.E. (Ed.). The new Wave, Proceedings of the special session on sustainable shrimp culture, Aquaculture. The World Aquaculture society, Baton Rouge, LA. USA, 2001, p.105-135.

FLEGEL, T.W. The special danger of viral pathogens in shrimp translocated for aquaculture. Science Asia v.32, p. 215-221, 2006.

HOSSAIN, Md.S. et al. Detection of WSSV in cultured shrimps, captured brooders, shrimp postlarvae and water samples in Bangladesh by PCR using different primers. Aquaculture v. 237, p. 59-71, 2004. DOI: https://doi.org/10.1016/j.aquaculture.2004.04.028

HURST, C.J.; GERBA, C.P.; CECH, I. Effects of environmental variables and soil characteristics on virus survival in soil. Applied of Environmental Microbiology v.40, p. 1067-1079, 1980. DOI: https://doi.org/10.1128/aem.40.6.1067-1079.1980

KUMAR, S.S. et al. Viability of white spot syndrome virus (WSSV) in sediment during sun-drying (drainable pond) and under non-drainable pond conditions indicated by infectivity to shrimp. Aquaculture v. 402–403, p. 119-126, 2013. DOI: https://doi.org/10.1016/j.aquaculture.2013.04.001

LIGHTNER, D.V. et al. Historic emergence, impact and current status of shrimp pathogens in the Americas. Journal of Invertebrate Pathology v. 110, p. 174–183, 2012. DOI: https://doi.org/10.1016/j.jip.2012.03.006

LO, C.F. et al. Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Disease of Aquatic Organisms v. 25, p. 133-141, 1996a. DOI: https://doi.org/10.3354/dao025133

LO, C.F. et al. White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Disease of Aquatic Organisms v.27, p. 215-225, 1996b. DOI: https://doi.org/10.3354/dao027215

MOHAN, C.V. et al. Vertical transmission of white spot baculovirus in shrimps – a possibility? Current Science v. 73, p. 109-110, 1997.

MOMOYAMA, K. et al. Cryopreservation of penaeid rod-shaped DNA virus (PRDV) and its survival in sea water at different temperatures. Fish Pathology v.33, n. 2, p. 95-96, 1998. DOI: https://doi.org/10.3147/jsfp.33.95

NATIVIDAD, K.D.; NOMURA, N.; MATSUMURA, M. Detection of White spot syndrome virus DNA in pond soil using a 2-step nested PCR. Journal of Virology Methods v.149, p. 28-43, 2008. DOI: https://doi.org/10.1016/j.jviromet.2008.01.013

OIE - OFFICE INTERNATIONAL DES EPIZOOTIES. Manual of diagnostic tests for Aquatic Animals. Disponível em: http://www.oie.int/index.php?id=2439&L=0&htmfile=chapitre_wsd.htm. Acesso em: 10 dezembro 2018.

QUANG, N.D. et al. Persistence of white spot syndrome vírus in shrimp ponds and surronding areas after outbreak. Environmental Monitoring and Assessment, p. 156-169, 2008. DOI: https://doi.org/10.1007/s10661-008-0463-7

SOTO, M.A.; SHERVETTE, V.R.; LOTZ, J.M. Transmission of white spot syndromevirus (WSSV) to Litopenaeus vannamei from infected cephalotorax, abdomen, or whole shrimp cadaver. Disease of Aquatic Organisms v.45, p.81-87, 2001. DOI: https://doi.org/10.3354/dao045081

VIJAYAN, K.K. et al. Polychaete worms – a vector for white spot syndrome virus (WSSV). Disease of Aquatic Organisms v.63, p. 107-111, 2005. DOI: https://doi.org/10.3354/dao063107

YAN, D.C. et al. White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp ponds sediments. Disease of Aquatic Organisms v.59, p. 69-73, 2004. DOI: https://doi.org/10.3354/dao059069

ZHUANG, J.; JIN, Y. Virus retention and transport as influenced by different forms of soil organic matter. Journal of Environmental Quality v. 32, p. 816-823, 2003. DOI: https://doi.org/10.2134/jeq2003.8160

Published
2019-01-07
How to Cite
CAVALLI, L. S.; BATISTA, C. R.; NORNBERG, B. F.; MARINS, L. F.; ABREU, P. C. Sediment and water can act as reservoirs for White spot syndrome virus in shrimp farming. Pesquisa Agropecuária Gaúcha, v. 25, n. 1/2, p. 1-7, 7 Jan. 2019.

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