EVALUATION OF AGRONOMIC TRAITS IN R, OAT POPULATION PRODUCED BY TISSUE CULTURE
Abstract
Tissue culture technique is been used to generate useful genetic mutation for breeding program. In this work it was evaluated the third generation of in vitro callus regenerated plant (R 3 somaclones). The assay was established in experimental field. It were seeded 19 somaclones of UPF 12 cultivar, 2 somaclones of CTC 2 cultivar as well as the controls. The experiment was carried out randomized with 3 repetitions of 1m 2 parcels with 250 seeds (5 rows with 50 seeds/row). It were analysed days to flowering, plant heigth, growth habit, test weight, 1000 seeds weight, grain yield and groat percentage. It was observed positive (useful) variation to these seven traits valued from UPF 12 tissue culture derived lines coMpared to the control. There were only negative variation for four characters. Hundred percent of evaluated UPF 12 somaclones showed . variation at least in one measured trait. The CTC 2 somaclones showed positive variation on three traits (days to flowering, test weight and 1000 seeds weight) and did not showed negativo alterations.
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